Serial registration analysis is compatible with both brightfield and fluorescent images and any of our HALO modules can be used for the final analysis step. The tumor regions, based off of the H&E classifier, are analyzed on the ER and HER2 slides using the Cytonuclear Module and Membrane Module, respectively. The serial section add-on takes the Classifier created from the H&E image and allows the user to apply the resulting classification mask to IHC-stained serial sections (e.g., ER, HER2) as regions of interest. A classifier has been created segmenting tumor from stroma (Tumor in red, stroma in green, glass in yellow). In the example above, a tissue biopsy section is stained with hematoxylin and eosin. Serial section analysis allows users to create a tissue classifier based off a reference slide (e.g., H&E or Pan-CK) and then apply the resulting classification mask to serial section(s) stained for additional IHC markers (e.g., HER2, ER, PR, Ki-67, CD8, CD4). The Serial Registration Analysis add-on includes options for both serial section analysis and serial stain fusion analysis.
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